A mouse was injected via tail vein with ~35 MBq (< 1mCi) of Tc-labeled human serum albumin (HSA). Imaging began immediately (~2 minutes) post-injection. Two acquisitions (one 16-minutes and one 32-minutes) were performed in succession. Projection data from the two acquisitions were combined (via the IVS Sum Projections tool). Eight frames were used for the gating. Mouse respiratory rate was stable and between 250-300 BPM for the duration of the acquisition.
Images courtesy of the National Cancer Institute, Amsterdam, Netherlands (NKI-AVL)
Longitudinal tumor study in a mouse using I131 (8d phys. half life, 364 keV peak) labeled NM404 (a radioiodinated phospholipid ether analog), imaged 20 hours (left) and 48 hours (right) post injection. At times of acquisition, as little as 11 MBq (300 µCi) and 6 MBq (160 µCi) were still present in the animal. The earlier acquisition took 60 the latter 72 minutes.
Despite the high energy of the I131 photons and the very low activity, there is only very little noise and a strong signal of the tumor visible in the images.
Male, NCR-Nude mouse bearing SKMEL3 tumor on left flank.
Special apertures used to shield high energy (365 keV) photons of I-131 and advanced reconstruction software used to produce artifact free, high-resolution images.
Injected dose only 87 µCi (3 MBq) of I-131 MIP-1145 i.v. as treatment of melanoma. Imaged 24 hours post injection, 80 minutes total acquisition time, due to very low activity.
This mouse was measured in the CT of a NanoSPECT/CT (45 kVp, 180 prj, 1s exposure, 6 minute acquisition) and then transferred to a small-animal PET system, where it was measured 30 minutes post injection of 322 µCi FDG in 110µl for 30 minutes (1 bed position).
Thanks to the dedicated Minerve animal handling system, which can be mounted on both systems, both modalities could be registered easily in the InVivoScope post-processing software.