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Show NanoSPECT studies: SPECT/CT

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SPECT-CT imaging of small peritoneal carcinomatosis.

		Comments
		We previously assessed the efficiency of intravenous injections of 125I-labeled mAbs for the treatment of small peritoneal carcinomatosis (Santoro et al, J. Nucl Med 2009). In the present study, athymic nude mice bearing intraperitoneal A-431 tumor cell xenografts expressing CEA receptors were intraperitoneally injected with 125I-35A7 mAb at days 4 following tumor cells xenograft. SPECT-CT imaging shows strong uptake of 125I-mAbs by tumors nodules, and demonstrates its usefulness for radioimmunotherapy of small solid tumors. Tracer details : The non-internalizing murine IgG1k 35A7 mAb, specific for the CEA Gold 2 epitope, was used to target CEA in transfected A-431 cells. Radiolabeling with iodine 125 was done according to the IODO-GEN method. Contributor : Jean-Pierre Pouget, IRCM
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Quintuple Image Co-Registration

		Comments
		A quadruple isotope SPECT/CT imaging acquisition was performed on a rat. 450uCi (17MBq) of Tc^99m-MDP, 1.4mCi (52MBq) of I¹²³, 600uCi (22MBq) of In¹¹¹, and 1mCi (37MBq) of Tl²⁰¹-Chloride were administered to the rat. A 40-minute image acquisition was performed at 3 hours post-injection of the Tc^99m/I¹²³ and 1 hour post-injection of the In¹¹¹/Tl²⁰¹. The color bars from left to right are: CT, Tc^99m, I¹²³, In¹¹¹ and Tl²⁰¹. Please see [158] for more details. Data courtesy of the University of New Mexico College of Pharmacy.
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Inflammation imaging in ferrets

		Comments
		A cohort of ferrets was induced with the flu in an attempt to visualize uptake of radiotracer in the sinuses. In this trial experiment 590uCi of In-111 (green) labeled with an inflammation targeting agent were injected. A fast, 7-minute CT acquisition was acquired over a 230mm scan range followed by a 20-minute SPECT acquisition. In-111 uptake in the trachea, heart, and lymphatic regions is clearly visible. Note also the striations visible in the ferret's trachea. Images courtesy of the University of New Mexico, College of Pharmacy, and the Lovelace Respiratory Research Institute.
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Dual-Isotope ECG-Gated SPECT/CT of a Rat

		Comments
		Dual-isotope ECG-gated SPECT/CT imaging was performed on a rat. 800uCi (30MBq) of Tc^99m-SestaMIBI and 970uCi (36MBq) of Tl²⁰¹-Chloride were injected into a rat. Data were acquired for 45 minutes at 60 minutes post-injection. Average heart rate was stable at ~320 BPM with the rat held under gas anesthesia. The image presents Tc^99m+CT data on the left and Tl²⁰¹+CT data on the right. Images courtesy of the University of New Mexico. [Tracer Details]
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Triple-isotope imaging of a mouse

		Comments
		SPECT/CT imaging of a mouse was performed following administration of three isotopes. 700uCi (26MBq) of Tc^99m-MDP, 1.2mCi (44MBq) of I¹²³, and 300uCi (11MBq) of Tl²⁰¹ were injected. Imaging was performed for 56 minutes at 60 minutes post-injection. MDP bone uptake is visualized in gray, I¹²³ uptake (red) is clearly visible in the thyroid, small intestine, and eyes, while Tl²⁰¹ (green) uptake is mostly evident in the kidneys. Images courtesy of The University of New Mexico College of Pharmacy.
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Demonstration of crosstalk correction between Tc-99m and I-123

		Comments
		Dual-isotope SPECT/CT imaging was performed on a rat following administration of Tc^99m-MDP (450uCi, 17MBq) and I¹²³ (1.4mCi, 52MBq). Image data were acquired for 60 minutes at 60 minutes post-injection. The image displays two reconstructions of the Tc^99m SPECT projection data. In the left-hand image, no corrections have been included to account for crosstalk of down-scattered I¹²³ photons into the Tc<up>99m energy window. Therefore, uptake in the thyroid and small intestine is clearly visible. In the right-hand image, a crosstalk correction method has been applied. The evidence of the I¹²³ distribution is eliminated without degradation of the reconstruction of the Tc^99m distribution. Images courtesy of The University of New Mexico, College of Pharmacy.
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Re-188 labeled oligomer (mouse, MIP)

		Comments
		Pretargeting has been attracting increasing attention as a drug delivery approach. We recently proposed Watson-Crick pairing of phosphorodiamidate morpholino oligomers (MORF) for the recognition system in tumor pretargeting. MORF pretargeting involves the initial i.v. injection of a MORF-conjugated antitumor antibody and the subsequent i.v. injection of the radiolabeled complement. Our laboratory has reported on MORF pretargeting for diagnosis using 99mTc as radiolabel. We now report on the use of MORF pretargeting for radiotherapy in a mouse tumor model using 188Re as the therapeutic radiolabel. For more details see: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1587615
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inviCRO Onion Model: Oncology

		Comments
		The results of an automated tumor segmentation of a mouse using inviCRO's digital scalpel powered by Definiens. The layered model or "onion" model is generated via topographical thinning or "peeling" of the segmented tumor. The four scenes are comprised of (left-to-right) an X-Ray CT (used to segment the tumor), the CT plus related SPECT data of the entire animal, the CT plus SPECT of the segmented tumor only (different color scale), and the CT plus inviCRO's onion model of the segmented tumor. This model, applicable to various imaging fields (see [148]), allows to analyze uptake within an organ. Special thanks to Ben Gershman, University of New Mexico and Dr. Jacob Hesterman, Bioscan for their support.
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inviCRO Onion Model: Respiratory analysis

		Comments
		A three-part animation displaying the results of an automated lung segmentation of a rat using the newly developed inviCRO digital scalpel powered by Definiens. The layered model or "onion" model of the lung is then generated via topographical thinning or "peeling" of the segmented lung. Application of this model to the image data allows for automated, quantifiable deposition of the relevant radiopharmaceutical in the lung. The three scenes are comprised of an X-Ray CT/Onion model fusion, SPECT/CT and lastly SPECT/Onion model.
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Aerosol lung deposition in a mouse (Volume rendering)

		Comments
		Approximately 150uCi (in 50uL) of stannous colloids labeled with Tc-99m were nebulized into the lungs of a normal FVB mouse with a microsprayer. Image data were acquired using a high-resolution, whole body multi-pinhole (9) aperture over 18 minutes, beginning approximately 15 minutes post-administration. A fully 3D volume rendering of the SPECT/CT data is shown. Images courtesy of Alain Le Pape, CNRS, Orleans, France
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